入驻第

购物车0
查看购物车已选择0个商品

在线交易:

在线客服

店内搜索

好友分享:

Thermo Scientific™ Pierce™ C18 Spin Columns




货号:89870

规格:25 COL/Pack

交货周期:现货1-2个工作日,期货现询

运杂费: 登录后可查看运杂费

DeepBio得分:5567.2
DeepBio得分 是基于文献引用次数,影响因子,文献新近度等因素计算的客观产品评级,得分越高表明该产品经过越可靠的实验验证,质量可信度越高

  • 产品详情
  • 评价
  • 包装清单
  • 售后服务

Description

Thermo Scientific Pierce C18 Spin Columns are ready-to-use centrifuge columns of porous C18 reverse-phase resin with excellent binding and recovery characteristics for peptide sample preparation for mass spectrometry and other methods.

Peptide samples can be purified and concentrated for a variety of applications using Pierce C18 Spin Columns. Each spin column contains a porous C18 reversed-phase resin with excellent binding and recovery characteristics for a wide range of peptide concentrations. The spin column format allows simultaneous processing of multiple samples (10 to 150 µL each) in approximately 30 minutes without laborious repeat pipetting or specialized equipment. Pierce C18 Spin Columns can be used effectively for processing peptides derived from 10 ng to 30 µg of protein. Sensitivity and detection limits are dependent on the downstream application.

Features of C18 Spin Columns:

• Removes interfering contaminants—significantly reduces signal suppression and improves signal-to-noise ratios and sequence coverage
• Simplifies optimization—processing yields high-quality spectra and is effective for a variety of reverse-phase-compatible contaminates
• Robust—works with a wide variety of load volumes and concentrations; no need to reduce sample volume before application
• Convenient—easy to handle and requires no special equipment to process multiple samples simultaneously (unlike tip-driven systems that require one sample to be processed at a time)
• Sensitive—special C18 resin allows excellent recovery percentages, even at low (sub-picomole) sample loads

Matrix-assisted laser desorption ionization (MALDI) and electrospray ionization (ESI) mass spectrometry (MS) are essential for studying biological compounds because of the high sensitivity and mass accuracy they provide. However, many of the buffers and compounds common to biological samples interfere with both MALDI-MS and ESI-MS, resulting in reduced sensitivity and poor-quality spectra. Pierce C-18 Spin Columns remove interfering contaminants and release peptides in MS-compatible solutions, providing an effective tool that aids in the MS examination of post-translational modifications or for the identification of proteins by peptide mapping.

Specifications

Bulk or Custom Form: Bulk Custom Form 1
Detection Method: Mass Spectrometry
Final Product: Peptide
For Use With (Equipment): Mass Spectrometer, Microcentrifuge
Product Line: Pierce™
Product Size: 25 columns
Starting Material: Peptides, Protease-digested protein
Workflow Step: Sample Clean Up

 

Contents & storage

Store at room temperature.