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Thermo Scientific™ SuperSignal™ West Femto Maximum Sensitivity Substrate




货号:34096

规格:200 mL

交货周期:现货1-2个工作日,期货现询

运杂费: 登录后可查看运杂费

DeepBio得分:5567.2
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Description

Thermo Scientific SuperSignal West Femto Maximum Sensitivity Substrate is an ultra-sensitive enhanced chemiluminescent (ECL) substrate for low femtogram protein level detection by western blot analysis with horseradish peroxidase (HRP) enzyme.

SuperSignal West Femto Maximum Sensitivity Substrate characteristics include:
• Sensitivity: low-femtogram (mid-zeptomole)
• Stability: 8-hr working solution stability, 1-year kit stability at 4°C
• Compatibility: nitrocellulose and PVDF membranes
• Signal duration: up to 8 hours
• Recommended primary antibody concentration: 1:5,000–1:100,000 dilution (10–200 ng/mL)
• Recommended secondary antibody concentration: 1:100,000–1:500,000 dilution (2–10 ng/mL)

Compare and learn more about our western blot chemiluminescent substrates ›
View all HRP substrates available ›

SuperSignal West Femto Maximum Sensitivity Substrate is designed for low femtogram-level detection by western blot analysis. When combined with optimized antibody concentrations and blocking buffers, SuperSignal West Femto substrate enables detection of target proteins in amounts that are too small to be seen with typical ECL substrates.

Specifications

Recommended Antibody Concentrations: 2°: 1:100-500K (2-10ng/mL), 1°: 1:5K (10- 200ng/mL)
Signal Duration: 6 to 8 hr
Detection Method: Chemiluminescent
Product Line: SuperSignal™
Product Size: 100 mL
Sensitivity: Low to mid-femtogram
Substrate Properties: Chemical Substrate
Substrate Type: HRP (Horseradish Peroxidase) Substrate

 

Contents & storage

Sufficient For: 12 mini-gel blots; 1000 cm2 of membrane
• Luminol/Enhancer, 50 mL
• Stable Peroxide Buffer, 50 mL