入驻第

购物车0
查看购物车已选择0个商品

在线交易:

在线客服

店内搜索

好友分享:

Pierce Silver Stain for Mass Spectrometry




货号:24600

规格:Kit

交货周期:现货1-2个工作日,期货现询

运杂费: 登录后可查看运杂费

DeepBio得分:5567.2
DeepBio得分 是基于文献引用次数,影响因子,文献新近度等因素计算的客观产品评级,得分越高表明该产品经过越可靠的实验验证,质量可信度越高

  • 产品详情
  • 评价
  • 包装清单
  • 售后服务

Description

Thermo Scientific Pierce Silver Stain for Mass Spectrometry is a complete kit for rapid and sensitive silver staining of proteins in polyacrylamide gels and efficient destaining of excised gel pieces for mass spectrometry analysis.

Features of Pierce Silver Stain for Mass Spectrometry :

• Sensitive and fast staining—the low-background, easy-to-use silver stain provides subnanogram sensitivity, detecting proteins at less than 0.25ng per spot in 30 minutes after fixing
• Flexible staining protocol—fix gel for 15 to 30 minutes or overnight without any affect on staining performance; stain for 1 to 30 minutes (typically 2 to 3 minutes)
• Robust—effective silver stain for even difficult-to-stain basic proteins, such as lysozyme (pI 10) and chymotrypsinogen A (pI 9.2), which are detectable at 0.2ng and 0.5ng, respectively
• Simple, trouble-free spot preparation—stained spots in excised gel-pieces are easily destained and made ready for tryptic digestion in one hour
• MS compatible—reagents and protocol are optimized to provide excellent performance in mass spectrometry following extraction of peptides from stained 1D or 2D gels (SDS-PAGE)
• Complete kit—contains all reagents necessary for staining and destaining before in-gel proteolysis and peptide recovery for analysis by mass spectrometry
• Convenient—kit-components are stable at room temperature; saves valuable refrigerator space and eliminates the need to equilibrate reagents before use

This silver staining kit for mass spectrometry (MS) bundles components of the high-performance Pierce Silver Stain Kit with optimized reagents to destain spots for subsequent in-gel tryptic digestion to recover peptide fragments for proteomics analysis. The resulting MS-compatible product and protocol deliver outstanding sensitivity and maintains favorable conditions for the high-yield recovery and identification (sequence coverage) of proteins by mass spectrometry. Silver staining of 2D gels is now an important intermediate step in a set of procedures that leads ultimately to identification of specific proteins in the proteome by mass fingerprinting methods. The Pierce Silver Stain protocol provides peak staining performance, flexibility, reliability and robustness for applications such as MALDI-MS.

The Pierce Silver Stain Kit for Mass Spectrometry enables both first-time and experienced users to achieve consistent and reliable staining using high-, low- and gradient-percentage polyacrylamide gels in single-dimension and 2D formats. The optimized staining method ensures extremely sensitive staining while minimizing covalent crosslinking of protein to the gel matrix, which can inhibit protein-peptide recovery following in-gel proteolysis. The destaining reagents facilitate complete removal of silver from stained protein bands and maximum protein recovery for subsequent mass spectrometry analysis.

Silver staining methods generally use either glutaraldehyde or formaldehyde, which cause some covalent crosslinking of protein to each other and the gel matrix. To the extent that this crosslinking occurs, extraction or elution of protein from the gel will be inhibited. Pierce Silver Stain uses formaldehyde in the stain and developer working solutions. However, the procedure accompanying the Kit for Mass 

Specifications

Target Molecule: Protein
Label or Dye: Silver
Detection Method: Colorimetric
Detection Location: In-Gel Detection
Product Line: Pierce™
Product Size: 1 L

Contents & storage

Kit Contents:
• Silver Stain Sensitizer, 2 mL
• Silver Stain Enhancer, 25 mL
• Silver Stain Developer, 500 mL
• Silver Stain, 500 mL
• Silver Destain Reagent A, 4 mL
• Silver Destain Reagent B, 14 mL

Storage: Upon receipt store at ambient temperature