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Thermo Scientific™ PageRuler™ Prestained Protein Ladder, 10 to 180 kDa




货号:26617

规格:10x250μl

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DeepBio得分:5567.2
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规格

Stain Type: Prestained
System: SDS-PAGE
Product Size: 10 x 250 µL

内容及储存

Upon receipt store at -20°C. Product is shipped with an ice pack

描述

Thermo Scientific PageRuler Prestained Protein Ladder is a mixture of ten (10) blue-, orange- and green-stained proteins (10 to 180 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.

Features of PageRuler Prestained Protein Ladder:

Size range—10 proteins spanning 10 to 180 kDa
Ready-to-use—supplied in a loading buffer for direct loading on gels; no need to boil
Sharp bands—color-coded bands of similar intensity for easy visualization
Quality tested—each lot evaluated by SDS-PAGE and Western blotting
Two reference bands—orange at 70kDa and green at 10kDa
Membrane-compatible—colored bands transfer to membranes for Western blotting

This prestained protein MW marker is designed for monitoring the progress of SDS-polyacrylamide gel electrophoresis, for assessing transfer efficiency onto PVDF, nylon and nitrocellulose membranes, and for estimating the approximate size of separated proteins that have been made visible with gel stains or Western blot detection reagents. The ladder contains one orange reference band at 70kDa and one green band at 10kDa.

Includes:
• Dye-stained proteins in 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3 and 33% glycerol.

Applications:
• Monitoring protein migration during SDS-polyacrylamide gel electrophoresis
• Monitoring protein transfer onto membranes after Western blotting
• Sizing of proteins on SDS-polyacrylamide gels and Western blots
For Research Use Only. Not for use in diagnostic procedures.

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