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Superdex 75 Increase 3.2/300




货号:29-1487-23

规格:见说明书

交货周期:现货2天,期货2-3周

运杂费: 登录后可查看运杂费

DeepBio得分:5567.2
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Superdex 75 Increase 3.2/300

Superdex 75 Increase 3.2/300 is a versatile, prepacked column for high resolution size exclusion chromatography (SEC) in micro scale (4 to 50 µL sample volume). The column is intended for preparative purification, characterization, and analysis of proteins with molecular weights between Mr 3000 and 70 000, such as recombinant tagged proteins. This is the column of choice when high resolution is required, sample amount is limited, and low buffer consumption is important.

  • Up to 50% higher resolution compared with predecessor Superdex 75, for improved purity and analysis results.
  • Runtime reduced down to one third compared with predecessor Superdex 75, with maintained resolution for results faster.
  • Versatile use in both preparative and analytical applications, especially with recombinant tagged proteins.
  • Tolerance to repeated harsh cleaning procedures at high pH ensures long column lifetime and minimizes carryover.
  • Higher lot-to-lot consistency compared with predecessor Superdex 75.

Higher resolution and/or reduced runtime compared with predecessor

Superdex 75 Increase belongs to the new generation of agarose based SEC media, comprising smaller, more rigid beads with a narrower particle size distribution and a higher selectivity for improved performance compared with its predecessor Superdex 75.

The column of choice for micro-scale preparative purification

The Superdex 75 Increase 3.2/300 column is well suited for micro-scale preparative purification such as the polishing step in a purification procedure, where µg amounts of highly size-homogenous target proteins can be obtained. The column is also suitable for characterization of proteins where high-quality data is required.

Clean in place for versatile use

Superdex 75 Increase resin is alkali-resistant. Hence, the column can be cleaned in place under alkali conditions to allow use of the same column for different proteins with minimal risk for carryover between samples. The alkali resistance is what differentiates agarose-based resins from silica-based resins. The possibility to clean the resin under harsh alkali conditions also ensures a long column lifespan.