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DYNABEADS MRNA PURIFICATION KIT




货号:61006

规格:1 KIT

计量单位:EA

交货周期:现货

运杂费: 登录后可查看运杂费

DeepBio得分:5567.2
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描述

The Dynabeads® mRNA Purification Kit rapidly isolates the mRNA transcriptome in typically 15 minutes, delivering pure, intact mRNA. The kit is designed to specifically target, capture, and purify mRNA molecules from total RNA preparations.
• Obtain pure mRNA in typically 15 minutes
• Recover and enrich the transcriptome efficiently
• Prepare mRNA that is suitable for nearly every downstream application
Pure, Rapid mRNA Isolation
The Dynabeads® mRNA Purification Kit contains magnetic beads for the isolation of the mRNA transcriptome from any total eukaryotic RNA preparation (Figure 1). Dynabeads® magnetic beads are uniform in size and highly mobile in solution (Figure 2). This enables the bead capture surface to quickly and continuously interact with the entire total RNA sample during the mRNA capture phase. The ribosomal RNA and small RNA molecules (transfer RNA, microRNA, small nucleolar RNA, and small cytoplasmic RNA) do not bind to the beads and are discarded. Only polyadenylated RNA species (mRNA) are captured resulting in cleaner, more sensitive results (Figure 3). Within about 15 minutes, pure mRNA is isolated and ready for use in downstream applications.

A Straightforward Enrichment Procedure
The Dynabeads® mRNA Purification Kit uses a robust affinity purification principle for the enrichment of polyadenylated mRNA. Superparamagnetic Dynabeads®, coupled to oligo-(dT)25, are first equilibrated with Binding Buffer, and then mixed with purified total RNA. The beads are then washed to remove contaminating RNA species, and then mRNA is eluted in as little as 5 µl of 10 mM Tris-HCl. The entire process is facilitated by the use of a neodymium magnet (purchased separately), which allows for the quick and efficient immobilization of the magnetic beads during buffer changes.

RNA is suitable for all downstream molecular applications including:
• Gene cloning 
• cDNA synthesis, cDNA library construction 
• RT-PCR, Quantitative RT-PCR 
• RPA (Ribonuclease Protection Assay) 
• Subtractive Hybridization 
• Dot/slot Hybridization 
• Primer extension 

For Research Use Only. Not for human or animal therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.

规格

Downstream Application: Cloning,Northern Blotting,Nuclease Protection Assays,Real-Time Quantitative PCR (qPCR),Reverse Transcriptase PCR (RT-PCR),cDNA Library Construction
Final Product: mRNA
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Isolation Technology: Magnetic Bead
Product Line: DYNAL®,Dynabeads®
Product Size: 2 mL
Sample Type (General): RNA
Sample Type (Specific): Total RNA
Shipping Condition: Room Temperature

内容及储存

2-8°C
2 ml Beads + Buffers
磁力架,磁珠,RNA,DNA纯化…购样品制备产品享双重礼!

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