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TransTaq® DNA Polymerase High Fidelity(HiFi)(含 2.5 mM dNTPs)




货号:AP131-13

CAS号:1

规格:6×500 units

交货周期:24h

运杂费: 登录后可查看运杂费

DeepBio得分:5567.2
DeepBio得分 是基于文献引用次数,影响因子,文献新近度等因素计算的客观产品评级,得分越高表明该产品经过越可靠的实验验证,质量可信度越高

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产品说明书


产品说明书 AP131

产品介绍

TransTaq® DNA Polymerase High Fidelity (TransTaq® HiFi DNA Polymerase)是以TransTaq®-T DNA Polymerase为主的复合酶TransTaq® HiFi DNA Polymerase具有5'→3' DNA聚合酶活性,5'→3'外切酶活性和3'→5'外切酶活性。不同模板使用不同Buffer实现高效扩增。TransTaq® HiFi Buffer I 适用于基因组DNA的扩增。TransTaq® HiFi   Buffer II  适用于 λDNA,cDNAPlasmid DNA的扩增。
• 保真性是EasyTaq® DNA Polymerase 18倍。
• 延伸速度为1-2 kb/min
• 扩增产物3' 端带"A"碱基,可直接克隆于pEASY®-T载体中。
• 基因组DNA片段的扩增 (≤15 kb)。

产品组成

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References

Li P, Du J, Goodier J L, et al. Aicardi–Gouti`eres syndrome protein TREX1 suppresses L1 and maintains genome integrity through exonuclease-independent 
ORF1p depletion[J]. Nucleic acids research, Nucleic Acids Res. 2017, 45(8):4619-4631.

Che L H, Zhang SQ, Li Y, et al. Genome-wide survey of nuclear protein-coding markers for beetle phylogenetics and their application in resolving both deep 
and shallow-level divergences[J]. Molecular Ecology Resources, 2017, 17(6):1342.

You CX , Zhao Q , Wang XF , et al. A dsRNA-binding protein MdDRB1 associated with miRNA biogenesis modifies adventitious rooting and tree architecture 
in apple[J]. Plant Biotechnology Journal, 2014, 12(2):183-192.

Gu P , Yang F , Kang J , et al. One-step of tryptophan attenuator inactivation and promoter swapping to improve the production of L-tryptophan in 
Escherichia coli[J]. Microbial Cell Factories, 2012, 11(1):30.

Wang G H , Sun B F , Xiong T L , et al. Bacteriophage WO Can Mediate Horizontal Gene Transfer in Endosymbiotic Wolbachia Genomes[J]. Frontiers in 
Microbiology, 2016, 7:1867.