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精氨酸酶测试盒




货号:BS-DARG-200

规格:200

交货周期:2-3天

运杂费: 登录后可查看运杂费

DeepBio得分:5567.2
DeepBio得分 是基于文献引用次数,影响因子,文献新近度等因素计算的客观产品评级,得分越高表明该产品经过越可靠的实验验证,质量可信度越高

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说明书下载DARG.rar

分类名称 : 酶活性测定,血液尿液化学 产品介绍 :

测定方法  比色法(520nm)定量测定精氨酸酶活性。

产品说明  精氨酸酶(L-精氨酸尿水解酶)广泛存在于动物和植物中。它主要存在于人体肝脏中,而在乳房、肾脏、睾丸、唾液腺表皮和红细胞中含量低。精氨酸酶催化精氨酸转化成鸟氨酸和尿素, 且是尿素循环的最后一步。精氨酸酶活性是临床诊断的关键指标,血液中精氨酸酶活性高意味着有肝功能损伤。精氨酸血症是由于精氨酸酶的缺乏,多见于常染色体隐性遗传疾病。在科研和新药开发中,简单、直接和自动化的精氨酸酶活性检测手段是最为理想的。本公司研制的精氨酸酶检测试剂盒为测定精氨酸酶活性提供了一个灵敏、方便的手段。测试原理是根据精氨酸酶反应中产生的尿素与色素原形成一种有色复合物,其颜色深浅与样本中精氨酸酶的活性对应成比例。

产品特点  测量敏感、准确:采用96孔板检测,能检测低至1U/L的精氨酸酶活性。简单、高通量:整个测试过程只需要在微量板中培养底物和样本,然后加入显色试剂反应15min即可。通过高通量检测手段,每天可测试上千份样本。

产品用途  1、可直接检测酶制剂、血清、血浆、组织培养等样品中精氨酸酶的活性。2、用于药品和药理学研究,检测药物对精氨酸酶活性的影响。

储藏条件 在室温下运输。精氨酸缓冲液、尿素标准品在-20℃保存,其它组分在2-8℃保存.

DESCRIPTION  ARGINASE (L-arginine ureohydrolase EC 3.5.3.1) is present in mammals and plants. In humans, arginase is expressed predominantly in the liver, and to lesser degrees in breast, kidney, testes, salivary glands, epidermis and erythrocytes. Arginase catalyzes the conversion of arginine to ornithine and urea, completing the last step in the urea cycle. Arginase activity is a key diagnostic indicator. Increased levels of arginase activity in blood have been associated with liver damage. Hyperargininemia due to arginase deficiency is an inherited autosomal recessive disease. Simple, direct and automation-ready procedures for measuring arginase activity in biological samples are highly desirable in Research and DrugDiscovery. Our arginase assay kit provides a sensitive and convenient method for arginase activity determination. The method utilizes a chromogen that forms a colored complex specifically with urea produced in the arginase reaction. The intensity of the color is directly proportional to the arginase activity in the sample.

KEY FEATURES  Sensitive and accurate. Detection limit: 1 U/L arginase activity in 96-well assay format. Simple and high-throughput. The procedure involves incubation of the provided substrate with the sample in a microplate, addition of the coloring reagent and incubation for 15 min. Can be readily automated as a high-throughput assay for thousands of samples per day.

APPLICATIONS  Direct Assays: arginase activity in enzyme preparations, serum, plasma, tissue culture etc; Drug Discovery/Pharmacology: effects of drugs on arginase activity.

STORAGE CONDITIONS  Kit is shipped at room temperature. Shelf life: at least 6 months.

其他 :

编号:DARG-048,DARG-200

测试:,48,200T